Top Tips for Observing Mitosis Lab

Top Tips for Observing Mitosis Lab We have all seen illustrations in textbooks of how mitosis works. While these types of diagrams are definitely beneficial for visualizing and understanding the stages of mitosis in eukaryotes and connecting them all together to describe the process of mitosis, it is still a good idea to show students how the stages actually look under a microscope in an actively dividing group of cells. Necessary Equipment for This Lab In this lab, there are some necessary equipment and supplies that would need to be purchased that go beyond what would be found in all classrooms or homes. However, most science classrooms should already have some of the necessary components of this lab and it is worth the time and investment to secure the others for this lab, as they can be used for other things beyond this lab. Onion (or Allum) root tip mitosis slides are fairly inexpensive and easily ordered from various scientific supplies companies. They can also be prepared by the teacher or students on blank slides with coverslips. However, the staining process for homemade slides are not as clean and exact as those that are ordered from a professional scientific supply company, so the visual may be somewhat lost. Microscope Tips Microscopes used in this lab do not have to be expensive or high powered. Any light microscope that can magnify at least 40x is sufficient and can be used to complete this lab. It is recommended that students are familiar with microscopes and how to use them correctly before beginning this experiment, as well as the stages of mitosis and what happens in them. This lab can also be completed in pairs or as individuals as the amount of equipment and skill level of the class allows. Alternatively, photos of onion root tip mitosis can be found and either printed onto paper or put into a slideshow presentation in which the students can do the procedure without the need for microscopes or the actual slides. However, learning to use a microscope properly is an important skill for science students to have. Background and Purpose Mitosis is constantly happening the meristems (or growth regions) of roots in plants. Mitosis occurs in four phases: prophase, metaphase, anaphase, and telophase. In this lab, you will determine the relative length of time each phase of mitosis takes in the meristem of an onion root tip on a prepared slide. This will be determined by observing the onion root tip under the microscope and counting the number of cells in each phase. You will then use mathematical equations to figure out time spent in each phase for any given cell in an onion root tip meristem. Materials Light microscope Prepared Onion Root Tip Mitosis Slide Paper Writing utensil Calculator Procedure 1.  Create a data table with the following headings across the top: Number of Cells, Percentage of all Cells, Time (min.); and the stages of mitosis down the side: Prophase, Metaphase, Anaphase, Telophase. 2.  Carefully put the slide on the microscope and focus it under low power (40x is preferred). 3.  Choose a section of the slide where you can clearly see 50-100 cells in the different stages of mitosis (each â€Å"box† you see is a different cell and the darker stained objects are chromosomes). 4.  For each cell in your sample field of view, determine whether it is in prophase, metaphase, anaphase, or telophase based on the appearance of the chromosomes and what they should be doing in that phase. 5.  Make a tally mark under the â€Å"Number of Cells† column for the correct stage of mitosis in your data table as you count your cells. 6.  Once you have finished counting and classifying all of the cells in your field of view (at least 50), calculate your numbers for â€Å"Percentage of All Cells† column by taking your counted number (from Number of Cells column) divided by the total number of cells you counted. Do this for all stages of mitosis. (Note: you will need to take your decimal you get from this calculation times 100 to make it into a percentage) 7.  Mitosis in an onion cell takes approximately 80 minutes. Use the following equation to calculate data for your â€Å"Time (min.)† column of your data table for each stage of mitosis:  (Percentage/100) x 80 8.  Clean up your lab materials as directed by your teacher and answer the analysis questions. Analysis Questions 1.  Describe how you determined which phase each cell was in. 2.  In which phase of mitosis was the number of cells the greatest? 3.  In which phase of mitosis was the number of cells the fewest? 4.  According to your data table, which phase takes the least amount of time? Why do you think that is the case? 5.  According to your data table, which phase of mitosis lasts the longest? Give reasons as to why this is true. 6.  If you were to give your slide to another lab group to have them repeat your experiment, would you end up with the same cell counts? Why or why not? 7.  What could you do to tweak this experiment in order to get more accurate data? Expansion Activities Have the class compile all of their counts into a class data set and recalculate the times. Lead a class discussion on the accuracy of data and why it is important to use large amounts of data when calculating in science experiments.

Advantages and disadvantages of outsourcing in IT Project Management Research Paper

Advantages and disadvantages of outsourcing in IT Project Management - Research Paper Example This research will begin with the statement that in most of the businesses today, the sole aim is to operate at optimum levels, where they reduce as many costs as possible and increase revenues to their best. In cost reduction, it is about reducing expenditure, especially expenditure, and ensuring that they use the cheapest products and services as possible. For instance, a company might be in need of a certain service for just but a short period of time. It could also be in need of such a service seasonally and not throughout. It would then be quite expensive and unnecessary to hire a full-time professional to handle that task for a short period then stay idle for a larger amount of time. In such cases, the company could consider hiring that professional on a contractual kind of a relationship. This is the primary concept lying behind outsourcing. Outsourcing is essentially the act of assigning certain business processes or activities to an external party that can help in handling t hem. Most of the time, large organizations cannot handle all their processes internally, either because they are too complex, bulky, or seasonal. The more common reason is the temporary nature of some tasks, such that the company is not willing to hire professionals permanently to carry out these activities from within. When the company then outsources the work to the external service provider, they accord them the responsibility of handling that activity all by themselves and maintaining the firm’s interests and assets at the same time.

The impact of graphic design on new media(Graphic Design) Dissertation

The impact of graphic design on new media(Graphic Design) - Dissertation Example The Internet, of course, has played a large part in this. Graphic design is intimately connected with new media – Engholm (2002) states that new media would not have risen to prominence as quickly as it did without graphic design. Therefore, the two concepts are interconnected. Because they are, it is important to discovery how one influences the other. In particular, this study seeks to find out how graphic design influences the consumers of new media. Especially important is the effect upon young people, because they are intimately connected. Young people have virtually always had new media in their lives, and, indeed, they tend to use new media to a profound level. New media is ubiquitous in their lives. To this end, the study focuses upon individuals who are purveyors of new media, yet, by and large, are not experts on graphic arts. They simply â€Å"like what they like.† Graphic arts might be a turnoff if not done correctly. Alternatively, it might be a draw for ot hers, if it is done right. What the study has found is that, with regards to websites, graphic arts are important, but content is more important. With advertisements, it seems to be the opposite of this. Aims and Objective The aim of this project is to show how graphic design and the new media interact. Another aim is to show how young people are influenced by graphic design in their websites and online advertisements. The objective is to tie new media, graphic design and their effects on young people to show graphic designers, advertisers and other purveyors of new media how they can reach the young audience with graphics. New media uses graphic design in a variety of ways. Advertisements, online video games and websites are all ways that graphic design and new media intersect. This study will focus on different ways that graphic design and the Internet intersect, and how graphic design is used on the Internet. This study will examine different online advertisements, web sites and online video game sites to determine how graphic design is used. This study will also examine how young people react to the graphics, and what can be done to make the graphics better.  Abstract The first chapter will give an overview of graphic arts and new media. The second chapter will go more into depth in these concepts. The third chapter will feature the results of the interviews and surveys done on the participants to this study. The fourth chapter will synthesize the findings and provide a road map for what should come next in the area of graphic design, as it relates to new media. The fifth chapter will conclude the paper.  Chapter 1 In this chapter, a road map for the dissertation will be offered. The first part of this chapter will concentrate on the overview of the graphic design and new media, providing a brief history and definition of the two concepts. The second part will give a brief overview of the literature survey. The third part will explain the research de sign, including the limitations of the particular design, as well as a brief overview